Spectrofluorimetric Determination and Validation of Biotin in Pure and Dosage Form via Derivatization with 4-Fluoro-7-nitrobenzofurazan
CHINESE JOURNAL OF CHEMISTRY, cilt.28, sa.11, ss.2209-2215, 2010 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 11
- Basım Tarihi: 2010
- Doi Numarası: 10.1002/cjoc.201090365
- Dergi Adı: CHINESE JOURNAL OF CHEMISTRY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.2209-2215
- İstanbul Üniversitesi Adresli: Hayır
Özet
A simple and highly sensitive spectrofluorimetric method was developed for the determination of biotin in pure and dosage form. The method is based on the derivatization of biotin with 4-fluoro-7-nitrobenzofurazan in borate buffer of pH 9.0 to yield a yellow, fluorescent product. The various chemical conditions that affected the reaction were studied. The method was validated for specificity, linearity, precision, accuracy, recovery and robustness. At optimized experimental conditions, a linear relationship between the fluorescence intensity of the concentration of biotin is observed in the range 45-450 ng/mL. Limit of detection and quantification were 0.038 and 0.114 ng/mL, respectively. The percentage mean recovery was 99.96. The proposed procedure was successfully applied to the determination of biotin in its dosage form with mean recovery of 101.23 +/- 1.22 for biotin tablets. The results obtained were in good agreement with those obtained by the reference method.