Nobel medicus, vol.18, no.1, pp.54-61, 2022 (Peer-Reviewed Journal)
Objective: Short tandem repeats (STRs) are short
sequences of nucleotides that are repeated and distributed
all over the genome. These polymorphisms enable
investigation of the forensic, ancestral lineage and
evolutionary studies in human population. Owing to the
historical migration and ethnic groups, it is very valuable
to evaluate genetic distances in Turkey. The aim of the
present study is to examine the STR data of Istanbul and
compare the genetic distances and allele frequency with
the previously published data of 27 countries from Europe,
Asia, America, Africa and Middle East.
Material and Method: Peripheral blood samples were
obtained from 400 healthy individuals. DNA samples
were amplified using a commercial kit. Multiplex STRPCR (Applied Biosystems, Foster City, CA, USA) was used
and the amplicons were evaluated on an ABI 3130 Genetic
Results: Among all loci, D21S11 and D18S51 were the
most polymorphic loci. The power of discrimination (PD)
ranged from 0.8329 (TPOX) to 0.9722 (D18S51). The
combined PD and probability of exclusion (PE) were found
to be >0.99999999 and 0.99999671, respectively.
Conclusion: In this study, six STR markers were selected
to compare the genetic distances and allele frequency of
the present results with the results of twenty-seven studies
which were published previously. This study indicates
that the population in Turkey is an intermediate between
Europe, Middle East and Central Asia.
Keywords: Human genetics, polymorphism, population
genetics, short tandem repeat.