Analysis of Virulence Factors and Antimicrobial Resistance in Salmonella Using Molecular Techniques and Identification of Clonal Relationships Among the Strains

Unlu O., Aktas Z., TUĞRUL H. M.

MICROBIAL DRUG RESISTANCE, cilt.24, sa.10, ss.1475-1482, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 24 Sayı: 10
  • Basım Tarihi: 2018
  • Doi Numarası: 10.1089/mdr.2018.0042
  • Dergi Adı: MICROBIAL DRUG RESISTANCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1475-1482
  • Anahtar Kelimeler: Salmonella enterica, gastroenteritis, virulence genes, pulsed-field gel electrophoresis, plasmid-based replicon typing, antimicrobial susceptibility, MULTIPLE ANTIBIOTIC-RESISTANCE, ENTERICA SEROVAR TYPHIMURIUM, ESCHERICHIA-COLI, DRUG-RESISTANCE, GENE CASSETTES, PLASMID, ENTERITIDIS, HUMANS, SEQUENCE, INTEGRON
  • İstanbul Üniversitesi Adresli: Evet

Özet

A total of 50 Salmonella enterica strains were isolated from clinical samples from 2009 to 2012 and analyzed for the presence of virulence genes found in SPI-1, SPI-2, and plasmids. The distribution and frequency of the antimicrobial resistance genes and plasmids were revealed, and pulsed-field gel electrophoresis (PFGE) patterns were investigated. Five genes were identified from the seven strains with resistance or intermediate resistance to ampicillin: blaSHV-1 (present in six strains), qnrS1 (present in five strains), blaTEM-1 (present in three strains), blaCTX-M-1 (present in one strain), and qnrB1 (present in one strain). One trimethoprim-sulfamethoxazole-resistant strain was positive for sulI but negative for sulII. In addition, we detected TEM-1 and qnrS1 in one strain; SHV-1 and qnrS1 in two strains; TEM-1, SHV-1, CTX-M-1, and qnrS1 in one strain; TEM-1, SHV-1, and qnrB1 in one strain; and SHV-1 and sulI genes in one strain together. Plasmid-based replicon typing assay revealed that all 50 strains carried FIIS, 13 carried I1, 1 carried I2, 4 carried P, 1 carried A/C, and 4 carried X1 replicon. PFGE was used to type 46 of the 50 strains and classify them into 22 major groups, 33 pulsotypes, and 8 major clusters. All strains carried all the virulence genes of interest on both Salmonella Pathogenicity Islands 1 and 2 and plasmids suggested high potential for pathogenicity. All antimicrobial-resistant strains contained at least one of the resistance genes of interest, confirming a phenotype-genotype association in antimicrobial resistance.