The smr gene resides on a novel plasmid pSP187 identified in a Staphylococcus pasteuri isolate recovered from unpasteurized milk


BJORLAND J., BRATLIE M. S. , Steinum T. M.

PLASMID, vol.57, no.2, pp.145-155, 2007 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 57 Issue: 2
  • Publication Date: 2007
  • Doi Number: 10.1016/j.plasmid.2006.08.004
  • Journal Name: PLASMID
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.145-155
  • Keywords: Staphylococcus pasteuri, multidrug resistance, smr gene, rolling-circle replication plasmid, RCR group VI, FtsK/SpoIIIE, QUATERNARY AMMONIUM-COMPOUNDS, COAGULASE-NEGATIVE STAPHYLOCOCCI, COMPLETE NUCLEOTIDE-SEQUENCE, BACILLUS-SUBTILIS SPOIIIE, RESISTANCE PLASMID, MOLECULAR CHARACTERIZATION, DISINFECTANT RESISTANCE, MEDIATES RESISTANCE, CRYPTIC PLASMID, DATABASE
  • Istanbul University Affiliated: No

Abstract

This work describes a novel plasmid pSPI 87 (5550 bp) carrying the small multidrug resistance determinant smr encoding resistance to quaternary ammonium compounds (QACs). pSP 187 was identified in a Staphylococcus pasteuri isolate recovered from bulk milk in a dairy cattle herd in Norway. Sequence analysis revealed 6 putative ORFs in addition to the smr gene within a cassette with identical genetic organization to that found in the pSK41-like Staphylococcus aureus plasmid pTZ22. A protein homology search suggested the gene product of ORF7 to be a putative replication initiation protein, while ORF2 was predicted to encode a protein homologous to members of FtsK/SpoIIIE cell division-DNA segregation protein families. Sequence similarities to some initiator proteins of rolling circle replicons (RCR) indicated that pSP187 uses a RCR mode of replication, supported by the detection of intermediate ssDNA using SI nuclease treatment and hybridization analysis. Interestingly, a 30-bp sequence found upstream from ORF7 showed high similarity to other dyad symmetry motifs proposed as putative double-strand origins of replication in the plasmids pG13 (Bacillus thuringiensis), pSTK1 (Bacillus stearothermophilus), and pERI-2 (Streptococcus thermophilus). In conclusion: The novel smr-containing plasmid pSP 187 is the first member of RCR group VI to be identified in a Staphylococcus sp. (c) 2006 Elsevier Inc. All rights reserved.