Kocatepe Veterinary Journal, cilt.13, sa.4, ss.383-387, 2020 (Hakemli Dergi)
In the present study we investigate the effects of parthenogenetic activation on in vitro embryo development and
quality in different activation periods. oocytes were obtained 14 hours after human chorionic gonadotropin
(hCG) injection from superovulated B6D2F1 female mice then parthenogenetic activation started 18 hours after
hCG injection. The oocytes were activated at different activation periods for 3, 4, 5 or 6 hours in 10 mM
strontium chloride (SrCl2)+ 5 μg/mL-1 Cytohalasine B (CB) + 5 nM Trichostatin A (TSA) containing a Ca 2+ free
Chatot Ziomek Brinster (CZB) activation medium, followed by further incubation for two hours at 37°C and 5%
CO2 in embryo culturing medium + TSA. The results in the present study suggested that the parthenogenetic
activation of the 6 hour activation period was found to be higher than at 3, 4 and 5 hours.