GC-MS Analysis and Antimicrobial Activity of Essential Oil of Stachys cretica subsp smyrnaea

Oeztuerk M., Duru M. E., Aydogmus-Oeztuerk F., Harmandar M., Mahhch M., Kolak U., ...More

NATURAL PRODUCT COMMUNICATIONS, vol.4, no.1, pp.109-114, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 4 Issue: 1
  • Publication Date: 2009
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.109-114
  • Keywords: Stachys cretica subsp smyrnaea, essential oil, trans-beta-caryophyllene, germacrene-D, antimicrobial activity, RADICAL SCAVENGING ACTIVITY, VOLATILE CONSTITUENTS, ENDEMIC VERBASCUM, MEDICINAL-PLANTS, AERIAL PARTS, TURKEY, ANTIBACTERIAL, LAVANDULIFOLIA, ANTIFUNGAL, GLYCOSIDES
  • Istanbul University Affiliated: Yes


The essential oil from the aerial parts of Stachys cretica L. subsp. smyrnaea Rech. fil. (Lamiaceae), endemic to Turkey, was investigated by using GC and GC-MS. Thirty-four of 37 components, represented 99.7% of the total oil, were identified. The major components of the essential oil were trans- beta-caryophyllene (51.0%,), germacrene-D (32.8%), alpha-humulene (3.1%), delta-cadinene (2.1%) and delta-elemene (2.1%). The antimicrobial activity of the essential oil, trans- beta-caryophyllene and five different extracts of the aerial parts of S. cretica L. subsp. smyrnaea were investigated by the standard disc diffusion method. The essential oil and trans-beta-caryophyllene exhibited antibacterial and antifungal activities. The activity increased with increasing concentrations of the essential oil and the extracts. The essential oil showed antimicrobial activity, particularly against Pseudomonas aeruginosa and Bacillus subtilis. The extracts exhibited either moderate or no activity.