Novel NLRP3/cryopyrin mutations and pro-inflammatory cytokine profiles in Behets syndrome patients


YÜKSEL Ş., Eren E., Hatemi G. , Sahillioglu A. C. , Gultekin Y., Demiroz D., ...Daha Fazla

INTERNATIONAL IMMUNOLOGY, cilt.26, ss.71-81, 2014 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 26 Konu: 2
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1093/intimm/dxt046
  • Dergi Adı: INTERNATIONAL IMMUNOLOGY
  • Sayfa Sayıları: ss.71-81

Özet

The role of mutations in NLRP3 in inflammatory features of Behets syndrome.Behets syndrome (BS) is a systemic inflammatory disorder with unknown etiology. Features of both innate and adaptive immunity have been claimed in the pathogenesis of BS. To test the possible dysregulation of the NLRP3/cryopyrin (Nod-like receptor with a pyrin domain 3) inflammasome, as a result of mutation(s), we performed single-strand conformation polymorphism analyses and/or sequencing of all the coding regions and intronexon boundaries of NLRP3/cryopyrin and ASC (apoptosis-associated speck-like protein containing CARD) genes from Turkish BS patients and healthy controls. At the same time, we determined pro-inflammatory cytokine secretion profiles of peripheral blood cells in response to LPS treatment using ELISA. BS patients with vascular involvement showed significantly increased levels of TNF- release at 2-, 4- and 8-h post-treatment and significantly increased IL-1 levels were detected at 2h (P 0.005) and 4h (P 0.025) (n 10). We identified four mutations in the NLRP3/cryopyrin gene, V200M (n 3/104) and T195M (n 1/104), in BS patients but none in control samples. No mutations were detected in the ASC gene. The effect of these NLRP3/cryopyrin mutants on ASC speck assembly and IL-1 secretion was tested and the V200M mutant was shown to induce IL-1 secretion. Thus, it is likely that certain mutations in NLRP3/cryopyrin in combination with yet unknown other factors may contribute to the pro-inflammatory cytokine profiles in BS patients.