Localization of P42 and F-1-ATPase alpha-Subunit Homolog of the Gliding Machinery in Mycoplasma mobile Revealed by Newly Developed Gene Manipulation and Fluorescent Protein Tagging

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Tulum I., Yabe M., Uenoyama A., Miyata M.

JOURNAL OF BACTERIOLOGY, cilt.196, sa.10, ss.1815-1824, 2014 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 196 Sayı: 10
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1128/jb.01418-13
  • Dergi Adı: JOURNAL OF BACTERIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1815-1824
  • İstanbul Üniversitesi Adresli: Hayır

Özet

Mycoplasma mobile has a unique mechanism that enables it to glide on solid surfaces faster than any other gliding mycoplasma. To elucidate the gliding mechanism, we developed a transformation system for M. mobile based on a transposon derived from Tn4001. Modification of the electroporation conditions, outgrowth time, and colony formation from the standard method for Mycoplasma species enabled successful transformation. A fluorescent-protein tagging technique was developed using the enhanced yellow fluorescent protein (EYFP) and applied to two proteins that have been suggested to be involved in the gliding mechanism: P42 (MMOB1050), which is transcribed as continuous mRNA with other proteins essential for gliding, and a homolog of the F-1-ATPase alpha-subunit (MMOB1660). Analysis of the amino acid sequence of P42 by PSI-BLAST suggested that P42 evolved from a common ancestor with FtsZ, the bacterial tubulin homologue. The roles of P42 and the F1-ATPase subunit homolog are discussed as part of our proposed gliding mechanism.