Akademik Veri Yönetim Sistemi
Journal of Drug Delivery Science and Technology, cilt.114, 2025 (SCI-Expanded)
Ocular infections are among the most prevalent health concerns worldwide, particularly following surgical procedures. Although various pharmaceutical formulations are available for their treatment, solution-based formulations remain the most common. However, these conventional solutions suffer from significant drawbacks, including short ocular residence time, low bioavailability, and the need for frequent administration throughout the day. To overcome these limitations, in situ gel systems offer distinct advantages due to their ability to transition from liquid to gel upon contact with physiological conditions, thereby prolonging drug retention and improving therapeutic outcomes. In the present study, a moxifloxacin (MFX)-loaded in situ gel formulation was developed and subjected to comprehensive characterization. The formulation was prepared using Poloxamer 188, Poloxamer 407, and methyl cellulose. The optimized system exhibited a gelation temperature of 32.80 ± 0.61 °C, a pH of 6.86 ± 0.17, a viscosity of 24.00 ± 4.00 cP at 4 °C and 2873 ± 11.55 cP at 32 °C, a gelation time of 4.85 ± 0.58 s, and a drug content of 99.33 ± 0.23 %. Rheological analysis confirmed that the formulation demonstrated non-Newtonian pseudoplastic flow behavior. Antimicrobial efficacy testing revealed that the developed in situ gel achieved a 2–8-fold reduction in bacterial counts against multiple strains compared to the corresponding MFX solution. In vitro cell culture studies indicated a potential contribution to wound healing, while the HET-CAM assay demonstrated the absence of irritant or corrosive effects. Overall, the developed moxifloxacin-loaded in situ gel exhibits favorable physicochemical, antimicrobial, and biocompatibility profiles, highlighting its promise as an effective therapeutic approach for the treatment of ocular infections.