Research Information System
Journal of Clinical Pediatric Dentistry, vol.50, no.1, pp.236-244, 2026 (SCI-Expanded, Scopus)
Background: Regenerative endodontic procedures (REPs) offer biologically based treatments for immature teeth but may result in tooth discoloration, compromising esthetic outcomes. This study aimed to evaluate the color stability of three commonly used materials in REPs—ProRoot MTA (PMTA), Biodentine, and NeoPutty—with or without blood contamination. Methods: A total of 96 extracted human maxillary anterior teeth were standardized and randomly assigned into eight experimental groups (n = 12), based on the material used and presence or absence of blood contamination. Two additional control groups (saline only and blood only) were included. REP was simulated following the American Association of Endodontists protocol, including canal preparation, calcium hydroxide disinfection, and placement of the test material at the cemento-enamel junction. Color measurements (∆E) were obtained using a VITA Easy Shade® spectrophotometer at baseline and after 1, 3, 6 and 12 months. Results: Statistical analysis revealed significant effects of both time and material type on color change (p < 0.05). After 12 months, the highest discoloration was observed in the PMTA/Blood group (∆E = 7.42 ± 0.56), while the lowest discoloration occurred in the Biodentine/Saline (∆E = 2.18 ± 0.43) and NeoPutty/Saline (∆E = 2.26 ± 0.51) groups. Blood contamination significantly increased discoloration across all materials, with PMTA demonstrating the least color stability. Conclusions: Blood contamination adversely affects the color stability of materials used in regenerative endodontic procedures. Biodentine and NeoPutty exhibited superior esthetic outcomes compared to PMTA and may be more suitable for use in anterior teeth where esthetics is a priority.