A peroxiredoxin antioxidant is encoded by a dormancy-related gene, Per1, expressed during late development in the aleurone and embryo of barley grains
PLANT MOLECULAR BIOLOGY, cilt.31, sa.6, ss.1205-1216, 1996 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 31 Sayı: 6
- Basım Tarihi: 1996
- Doi Numarası: 10.1007/bf00040837
- Dergi Adı: PLANT MOLECULAR BIOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1205-1216
- Anahtar Kelimeler: late embryogenesis-abundant (lea), thiol-specific antioxidant (TSA), Hordeum vulgare L, desiccation tolerance, THIOL-SPECIFIC ANTIOXIDANT, ALKYL HYDROPEROXIDE REDUCTASE, VULGARE L SEEDS, AVENA-FATUA, HYDROGEN-PEROXIDE, GIBBERELLIC-ACID, SALMONELLA-TYPHIMURIUM, MOLECULAR-CLONING, OXIDATIVE DAMAGE, MULTIGENE FAMILY
- İstanbul Üniversitesi Adresli: Hayır
Özet
Antioxidants can remove damaging reactive oxygen species produced as by-products of desiccation and respiration during late embryogenesis, imbibition of dormant seeds and germination. We have expressed a protein, PER1, encoded by the Balem (barley aleurone and embryo) transcript previously called B15C, and show it to reduce oxidative damage in vitro. PER1 shares high similarity to a novel group of thiol-requiring antioxidants, named peroxiredoxins, and represents a subgroup with only one conserved cysteine residue (l-Cys). PER1 is the first antioxidant belonging to the l-Cys subgroup shown to be functionally active, and the first peroxiredoxin of any kind to be functionally described in plants. The steady state level of the transcript, Per1, homologous to a dormancy-related transcript (pBS128) from bromegrass (Bromus secalinus), increases considerably in imbibed embryos from dormant barley (Hordeum vulgare L.) grains. Our investigations also indicate that Per1 transcript levels are dormancy-related in the aleurone layer of whole grains. In contrast to most seed-expressed antioxidants Per1 disappears in germinating embryos, and in the mature aleurone the transcript is down-regulated by the germinating embryo or by gibberellic acid (GA). Our data show that the barley seed peroxiredoxin is encoded by a single Per1 gene. Possible roles of the PER1 peroxiredoxin in barley grains during desiccation, dormancy and imbibition are discussed.