Extractive-spectrophotometric determination of amine-type stimulants and antidepressants with anionic indicator dyes

Ercag E., Sarioglu G., Uzer A., Bora T., Apak R.

JOURNAL OF ANALYTICAL CHEMISTRY, vol.68, no.7, pp.583-589, 2013 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 68 Issue: 7
  • Publication Date: 2013
  • Doi Number: 10.1134/s1061934813070046
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.583-589
  • Istanbul University Affiliated: Yes


Amphetamines having a stimulant effect on the central nervous system, certain antidepressants, and slimming agents containing sibutramine bear the cationic amine constituents that can form colored ion-association complexes with anionic basic dyes (i.e. bromocresol purple (BCP), methyl orange (MeO), and eriochrome black-T (EBT)), and be extracted into organic solvents for spectrophotometric determination. The limit of detection (LOD, mg/L) of the tested amines varied within the following intervals: MDMA: 0.09-0.33; MDA: 0.14-1.43; fluoxetine: 0.09-0.24; sibutramine: 0.07-0.28, depending on the dye. The proposed rapid and cost-effective extractive-photometric method for MDMA, fluoxetine, and sibutramine was statistically validated against HPLC using ecstasy, prozac, and lida pharmaceutical tablets.