Oxidative damage to nuclear DNA in hyperthyroid rat liver: Inability of vitamin C to prevent the damage


Andican G. , Gelisgen R. , Civelek S. , Seven A. N. , Seymen O. , Altug T. , ...Daha Fazla

JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, cilt.67, ss.413-420, 2004 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 67 Konu: 5
  • Basım Tarihi: 2004
  • Doi Numarası: 10.1080/15287390490273479
  • Dergi Adı: JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES
  • Sayfa Sayıları: ss.413-420

Özet

The effects of hyperthyroidism on oxidative DNA damage in liver tissue and modification by vitamin C supplementation were investigated in rats. Animals were rendered hyperthyroid by administration of L-thyroxine (0.4 mg/100g food) for 25 d. In the plasma samples, T-3, T-4, and thyroid-stimulating hormone (TSH) were measured by radioimmunoassay and ascorbate spectrophotometrically. Oxidative damage to hepatic nuclear DNA was determined by measuring deoxy-guanosine (dG) and 8-oxodG by high-performance liquid chromatography with diode array detector electrochemical detection (HPLC-DAD-ECD). In hyperthyroidism, 8-oxodG/(10(5) dG) levels were significantly higher and plasma vitamin C levels lower than in control rats. The results of this experimental study show that oxidative damage to hepatic nuclear DNA increases in the hyperthyroid state and that vitamin C was not effective in preventing this damage.