Akademik Veri Yönetim Sistemi
Microscopy Research and Technique, 2024 (SCI-Expanded)
In this study, in vitro grown Aloe vera L. tissues were used for AgNP synthesis. Adventitious root and callus tissues were grown in MS medium containing 1 mg/L IAA and 1 mg/L NAA. Using A. vera L. leaf, in vitro grown callus, and adventitious roots tissue extracts, AgNPs were synthesized. According to DLS analysis, PDI values and zeta potential values showed that AgNPs from adventitious root were more suitable in terms of size and surface charge. Characterization of adventitious root-derived AgNPs was performed by UV–Vis absorption spectrum, ICP/MS, SEM, FTIR, and XRD. According to HPLC results, catechin, gentisic acid, caffeic acid, coumaric acid, polydatin, coumarin, and ellagic acid were found in adventitious roots. Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC2 7853), MRSA (ATCC 33951) and Staphylococcus aureus (ATCC 6538) strains were used to determine the antibacterial and antibiofilm activity of AgNPs. The highest antibacterial activity was determined against P. aeruginosa. Lower concentrations of AgNPs caused changes in the structure of the biofilm formed by P. aeruginosa, which produced particularly strong biofilms, resulting in failure of biofilm maturation. Accordingly, AgNPs synthesized from Aloe vera L. adventitious roots had antibacterial and antibiofilm activity even at low concentrations against the tested bacterial strains.