RP-HPLC METHOD FOR DETERMINATION OF OSELTAMIVIR PHOSPHATE IN CAPSULES AND SPIKED PLASMA

Aydogmus Z., Caglar S., Toker S.

ANALYTICAL LETTERS, cilt.43, sa.14, ss.2200-2209, 2010 (SCI-Expanded, Scopus)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 43 Sayı: 14
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1080/00032711003698721
  • Dergi Adı: ANALYTICAL LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.2200-2209
  • Anahtar Kelimeler: 4-Chloro-7-nitrobenzofurazan, Drug analysis, HPLC, Oseltamivir phosphate, Plasma, INFLUENZA, NEURAMINIDASE, TAMIFLU(R), VALIDATION, CHROMATOGRAPHY, CARBOXYLATE, METABOLITE, INHIBITOR, URINE, ASSAY
  • İstanbul Üniversitesi Adresli: Evet

Özet

A new, sensitive RP-HPLC method was developed for the determination of oseltamivir phosphate in capsules and plasma. The method was based on the reaction of the drug with 4-chloro-7-nitrobenzofurazan in borate buffer solution of pH 8.50. Isocratic chromatography was performed on a C(18) column with acetonitrile-10 mM nitric acid (pH 3, 60 + 40, v/v) as the mobile phase with fluorescence detection (lambda ex: 470 nm, lambda em: 541 nm). Mexiletine hydrochloride was used as an internal standard. Analytical parameters were evaluated. The calibration range was linear from 50.0-750.0 ng/ml. The mean percentage recovery in capsules and plasma were 99.95% and 95.42%, respectively.
RP-HPLC METHOD FOR DETERMINATION OF OSELTAMIVIR PHOSPHATE IN CAPSULES AND SPIKED PLASMA
Author(s): Aydogmus, Z (Aydogmus, Zeynep)[ 1 ] ; Caglar, S (Caglar, Sena)[ 1 ] ; Toker, S (Toker, Sidika)[ 1 ]
Source: ANALYTICAL LETTERS  Volume: 43   Issue: 14   Pages: 2200-2209   DOI: 10.1080/00032711003698721  Published: 2010
Times Cited: 3 (from Web of Science)
Cited References: 18      [ view related records ]     Citation MapCitation Map     
Abstract: A new, sensitive RP-HPLC method was developed for the determination of oseltamivir phosphate in capsules and plasma. The method was based on the reaction of the drug with 4-chloro-7-nitrobenzofurazan in borate buffer solution of pH 8.50. Isocratic chromatography was performed on a C(18) column with acetonitrile-10 mM nitric acid (pH 3, 60 + 40, v/v) as the mobile phase with fluorescence detection (lambda ex: 470 nm, lambda em: 541 nm). Mexiletine hydrochloride was used as an internal standard. Analytical parameters were evaluated. The calibration range was linear from 50.0-750.0 ng/ml. The mean percentage recovery in capsules and plasma were 99.95% and 95.42%, respectively.
Accession Number: WOS:000280892300008