Investigation of the Relationship Between Oxidative Stress and Glucose Signaling in Schizosaccharomyces pombe

Palabiyik B., Kig C., Pekmez M., Dalyan L., Arda N., Temizkan G.

BIOCHEMICAL GENETICS, vol.50, no.5-6, pp.336-349, 2012 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 50 Issue: 5-6
  • Publication Date: 2012
  • Doi Number: 10.1007/s10528-011-9477-x
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.336-349
  • Keywords: Schizosaccharomyces pombe, Glucose signaling, Oxidative stress response, Glucose repression, Fission yeast, PROTEIN-KINASE-A, FISSION YEAST, FBP1 GENE, SACCHAROMYCES-CEREVISIAE, SUPEROXIDE-DISMUTASE, CALORIE RESTRICTION, HYDROGEN-PEROXIDE, LIFE-SPAN, EXPRESSION, RESPONSES
  • Istanbul University Affiliated: Yes


The invertase mutant defective in the glucose signaling pathway of () is resistant to glucose repression. This mutant is able to consume sucrose alongside glucose and grows in glucose-containing media with a generation time close to that of the wild type. Intracellular oxidation, protein carbonyl, and reduced glutathione levels and catalase, superoxide dismutase, and glutathione peroxidase activity were investigated in , to determine the relationship between oxidative stress response and glucose signaling. The expression profiles of some genes involved in regulation of glucose repression ( fructose-1,6-bis-phosphatase; hexokinase) and stress response ( and transcription factors; catalase; Cu,Zn superoxide dismutase) were analyzed using the quantitative real-time PCR technique. Oxidative stress response in seems to be affected by glucose signaling in a manner different from that caused by glucose deprivation.