Investigation of the Relationship Between Oxidative Stress and Glucose Signaling in Schizosaccharomyces pombe

Palabiyik B., Kig C., Pekmez M., Dalyan L., Arda N., Temizkan G.

BIOCHEMICAL GENETICS, cilt.50, sa.5-6, ss.336-349, 2012 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 50 Sayı: 5-6
  • Basım Tarihi: 2012
  • Doi Numarası: 10.1007/s10528-011-9477-x
  • Dergi Adı: BIOCHEMICAL GENETICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.336-349
  • Anahtar Kelimeler: Schizosaccharomyces pombe, Glucose signaling, Oxidative stress response, Glucose repression, Fission yeast, PROTEIN-KINASE-A, FISSION YEAST, FBP1 GENE, SACCHAROMYCES-CEREVISIAE, SUPEROXIDE-DISMUTASE, CALORIE RESTRICTION, HYDROGEN-PEROXIDE, LIFE-SPAN, EXPRESSION, RESPONSES
  • İstanbul Üniversitesi Adresli: Evet

Özet

The invertase mutant defective in the glucose signaling pathway of () is resistant to glucose repression. This mutant is able to consume sucrose alongside glucose and grows in glucose-containing media with a generation time close to that of the wild type. Intracellular oxidation, protein carbonyl, and reduced glutathione levels and catalase, superoxide dismutase, and glutathione peroxidase activity were investigated in , to determine the relationship between oxidative stress response and glucose signaling. The expression profiles of some genes involved in regulation of glucose repression ( fructose-1,6-bis-phosphatase; hexokinase) and stress response ( and transcription factors; catalase; Cu,Zn superoxide dismutase) were analyzed using the quantitative real-time PCR technique. Oxidative stress response in seems to be affected by glucose signaling in a manner different from that caused by glucose deprivation.