The effects of N(1)-2,4-dihydroxybenzylidene-N-(4) hydroxybenzylidene-S-methyl-thiosemicarbazidato-oxovanadium(IV) on testicular damage in streptozotocin-induced diabetic rats


TUNALI S., BAL DEMİRCİ T., ÜLKÜSEVEN B., YANARDAĞ R.

JOURNAL OF RESEARCH IN PHARMACY, vol.26, no.3, pp.675-686, 2022 (ESCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 26 Issue: 3
  • Publication Date: 2022
  • Doi Number: 10.29228/jrp.165
  • Journal Name: JOURNAL OF RESEARCH IN PHARMACY
  • Journal Indexes: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Page Numbers: pp.675-686
  • Keywords: Diabetes mellitus, oxovanadium complex, testicular damage, oxidative stress, VANADYL SULFATE, ANTIOXIDANT ACTIVITY, OXIDATIVE STRESS, PARAMETERS, MECHANISM, APOPTOSIS, PROTECTS, INJURY
  • Istanbul University Affiliated: No

Abstract

Diabetes mellitus (DM) is a serious metabolic disorder that has negative effects on male sexual and reproductive functions in humans and animals. The purpose of current research is to demonstrate the effect of N(1)-2,4-dihydroxybenzylidene-N(4)-2-hydroxybenzylidene-S-methyl-thiosemicarbazidato-oxoyanadium(IV) (VOL) on testicular damage in male rats with streptozotocin (STZ)-induced diabetes. Male Swiss albino rats were randomly grouped as follows: Control (intact) group animals; control group animals given VOL (0.2 mM/kg/day) for 12 days; STZ-induced diabetic animals; diabetic animals given VOL group, at same dose and time. Experimental diabetes was induced with a single dose of 65 mg/kg intraperitoneal STZ injection. On day 12, overnight fasted animals were sacrificed and testis tissues (right and left) were collected and homogenized in 0.9 % saline. After centrifugation, protein levels and non-enzymatic parameters such as glutathione, lipid peroxidation, protein carbonyl, as well as the activities of alkaline phosphatase, myeloperoxidase and enzymatic antioxidants were determined. Based on the results obtained, VOL was shown to be a potentially beneficial compound in the amelioration of damaged testicular tissue of male diabetic rats after 12 days of administration. Our results suggest that VOL may be a promising candidate for the development of new generation antidiabetic drugs, and its administration to diabetic rats may be a suitable candidate in reducing testicular damage.