The AtMYB12 activation domain maps to a short C-terminal region of the transcription factor

Stracke R., Turgut-Kara N., Weisshaar B.

ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES, vol.72, pp.251-257, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 72
  • Publication Date: 2017
  • Doi Number: 10.1515/znc-2016-0221
  • Journal Name: ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.251-257
  • Keywords: activation domain, flavonol regulator, MYB12, production of flavonol glycosides, transcription factor, BIOSYNTHETIC-PATHWAY, FLAVONOL SYNTHESIS, ARABIDOPSIS, ACCUMULATION, EXPRESSION, MUTANTS, COMPLEX, ACID
  • Istanbul University Affiliated: Yes

Abstract

The Arabidopsis thaliana R2R3-MYB transcription factor MYB12 is a light-inducible, flavonol-specific activator of flavonoid biosynthesis. The transactivation activity of the AtMYB12 protein was analyzed using a C-terminal deletion series in a transient A. thaliana protoplast assay with the goal of mapping the activation domain (AD). Although the deletion of the last 46 C-terminal amino acids did not affect the activation capacity, the deletion of the last 98 amino acids almost totally abolished transactivation of two different target promoters. A domain swap experiment using the yeast GAL4 DNA-binding domain revealed that the region from positions 282 to 328 of AtMYB12 was sufficient for transactivation. In contrast to the R2R3-MYB ADs known thus far, that of AtMYB12 is not located at the rearmost C-terminal end of the protein. The AtMYB12 AD is conserved in other experimentally proven R2R3-MYB flavonol regulators from different species.