Objectives: In many studies, blood brain barrier has been shown
to be compromised in multiple sclerosis patients. Pericytes play
an active role in ensuring the continuity of the blood brain barrier
along with a series of cells. In this study, the effect of pericytic dysfunction on the development of demyelinating plaques in patients
with multiple sclerosis was investigated.
Material and Method: Concentrations of pericyte dysfunction
mediators (PDGFbb, MMP9, TIMP3 and ADAM17) in cerebrospinal
fluid of patients with clinically isolated syndrome (CIS), relapsing
remitting multiple sclerosis (RRMS) and healthy control group
were measured by ELISA and oligoclonal bands (OCB) were investigated. We aimed to determine whether the concentration of these
mediators differed between groups and whether they correlated
with lesion load, number of attacks, and EDSS scores.
Results: Concentrations of all four mediators were similar in patients with CIS and RRMS. However, both groups were found to
be higher than the healthy group. In the CIS and RRMS groups,
the levels of the mediators were not correlated with any parameters examined. However, the levels of PDGFbb (p=0.045), MMP9
(p=0.037), and TIMP3 (p=0.033) were higher in OCB positive
patients than in those without OCB, whereas ADAM17 levels remained unchanged.
Conclusion: This study shows that pericytes may play a role in the
pathogenesis of MS from early stages of the disease. The presence
of higher levels in patients with OCB suggests that pericyte dysfunction may be associated with OCB formation.
Keywords: Multiple sclerosis, clinically isolated syndrome, pericyte, cerebrospinal fluid, neuroinflammation