In this study, presence of superficial dermatomyces and Candida have been analysed on fungus infected suspicious dogs which are fed at various shelters brought to the Istanbul University Department of Microbiology and special clinics. The analyis has been performed with reference to the macrodilution method suggested at the NCCLS M38-A document and effect of fluconazole, ketoconazole, itraconzole, terbinafine, griseofulvin and miconazole have been analysed on the isolated agents. For this purpose, samples of skin scrapings and hairs have been collected from 85 dogs with skin lesion. After the mycologic analysis of the samples, 24 M. canis and one M. nanum strain have been isolated. It has been determined as a conclusion of this study that the dominant superficial dermatophyte species in dogs is M. canis and that terbinafine is the most effective antifungal against Microsporum species and hence considered an alternative for the azoles used in treatments.