HPLC fluorescence method for the determination of nizatidine in human plasma and its application to pharmacokinetic study


Cakar M. B. , Ulu S.

LUMINESCENCE, cilt.29, ss.357-361, 2014 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 29 Konu: 4
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1002/bio.2552
  • Dergi Adı: LUMINESCENCE
  • Sayfa Sayıları: ss.357-361

Özet

A sensitive high-performance liquid chromatographic (HPLC) method was developed for the determination of nizatidine in human plasma. Nizatidine was derivatized by 4-fluoro-7-nitrobenzofurazan (NBD-F). Chromatographic separation was performed on a Inertsil C-18 column (150 mm x 4.6 mm, 5 mu m) using isocratic elution by a mobile phase consisting of methanol/water (55:45) at a flow rate of 1.2 mL/min. Amlodipine was used as the internal standard (IS). Fluorescence detector was used operated at 461 nm (excitation) and 517 nm (emission), respectively. The calibration curve was linear over the range of 50-2000 ng/mL. This method was successfully applied to a pharmacokinetic study after oral administration of a dose (150 mg) of nizatidine. Copyright (c) 2013 John Wiley & Sons, Ltd.