Research Information System
33rd International Symposium on Microscale Separations and Bioanalysis (MSB 2017), Leiden, Netherlands, 26 - 29 March 2017, vol.13, pp.5-10, (Full Text)
In this assay, a selective, simplistic and sensitive new online heart-cutting Liquid Chromatographic
analysis method was developed for the quantification of linezolid in human serum. The new
method allows to the quantification of linezolid in serum without complicated sample preparation. Before
analysis, the samples were mixed with methanol and filtered. Separations were carried out with following
systems; 1st dimension is a system consisting of 2.1 x 50 mm Biphenyl pre-separation column with
acetonitrile and 0.03 M o-phosphoric acid solution mixture (15:85) as mobile phase and the 2nd dimension
is a system consisting of 4.6 x 150 mm Pentafluorophenyl propyl separating column with acetonitrile
and 0.03 M o-phosphoric acid solution mixture (25:75) as mobile phase. Two systems were connected via
a 6-port valve having 0.2 mL loop and separations were carried out continuously during injections. For
both systems, 250 nm has been selected as detection wavelength. The concentration range of 0.5-20
μg/mL was found as linear calibration curve. The new method was successfully applied for the
quantification of linezolid in human serum samples collected from a volunteer who has received 600 mg
linezolid orally.