Akademik Veri Yönetim Sistemi
REVUE ROUMAINE DE CHIMIE, cilt.68, sa.10-12, ss.527-532, 2023 (SCI-Expanded)
An ultra fast liquid chromatographic (UFLC) method using fluorimetric detection for the quantitation of Febuxostat (FEB) in human serum was developed by precolumn derivatization with 4bromomethyl-7-methoxy coumarin (BrMmC). The derivatization was catalyzed with dibenzo-18-crown-6-ether to produce a fluorescent derivative which was determined through fluorescence measurement with excitation at 320 nm and emission at 395 nm wavelengths. A C18 column with 100 cm x 4.6 mm, 3 mu m A calibration curve was then plotted as 0.005-3.0 mu g/mL with a detection limit of 0.0022 mu g/mL and quantification limit of 0.0073 mu g/mL. The mean recovery to reveal the accuracy and relative standard deviation (RSD) that indicates the precision of the method were found out to be 85.20 % and less than 3.52 %, respectively. The utility of the new method has been demonstrated by measuring the pharmacokinetics of FEB by administration of 80 mg tablets to a healthy female volunteer, aged 42.