PHARMACOKINETIC ANALYSIS OF FEBUXOSTAT IN HUMAN SERUM BY UFLC BASED ON FLUORIMETRIC DERIVATIZATION
REVUE ROUMAINE DE CHIMIE, vol.68, no.10-12, pp.527-532, 2023 (SCI-Expanded, Scopus)
- Publication Type: Article / Article
- Volume: 68 Issue: 10-12
- Publication Date: 2023
- Doi Number: 10.33224/rrch.2023.68.10-12.04
- Journal Name: REVUE ROUMAINE DE CHIMIE
- Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Page Numbers: pp.527-532
- Istanbul University Affiliated: No
Abstract
An ultra fast liquid chromatographic (UFLC) method using fluorimetric detection for the quantitation of Febuxostat (FEB) in human serum was developed by precolumn derivatization with 4bromomethyl-7-methoxy coumarin (BrMmC). The derivatization was catalyzed with dibenzo-18-crown-6-ether to produce a fluorescent derivative which was determined through fluorescence measurement with excitation at 320 nm and emission at 395 nm wavelengths. A C18 column with 100 cm x 4.6 mm, 3 mu m A calibration curve was then plotted as 0.005-3.0 mu g/mL with a detection limit of 0.0022 mu g/mL and quantification limit of 0.0073 mu g/mL. The mean recovery to reveal the accuracy and relative standard deviation (RSD) that indicates the precision of the method were found out to be 85.20 % and less than 3.52 %, respectively. The utility of the new method has been demonstrated by measuring the pharmacokinetics of FEB by administration of 80 mg tablets to a healthy female volunteer, aged 42.