VISCEA-Translational Cereal Genomics, Viyana, Austria, 9 - 11 February 2014, pp.33
Since domestication ~10,000 years ago, rice has become the most important grain for human nutrition and caloric intake worldwide. Years of breeding have resulted in many cultivars, yet continued improvement is needed for sustainable food security. For the past 50 years the FAO/IAEA laboratory has conducted research and development in plant mutation breeding. One current focus is the improvement of grain quality in rice for which we have designed an approach in which induced mutations and “omics” technologies are integrated. Near Infrared Reflectance Spectroscopy, proteome and metabolome analyses were carried out on M5 seed. Preliminary results show a high variation in NIRS spectra as well as 2D gel images suggesting many protein alterations in studied mutants. No statistical differences of profiled metabolites were observed among studied plants. Selected mutants are being further characterized using genome sequencing approaches to identify nucleotide variation.