Garcinia cambogia (malabar tamarind, bitter kola) is seen abundantly in the evergreen forests of Konkan in South India Hydroxycitric acid, mainly obtained from Garcinia cambogia, was identified by the end of the 1960's as a strong competitive inhibitor of the extramitochondrial enzyme adenosine triphosphate citrate lyase. The objective of this study was to present the effect of Garcinia cambogia extract known as weakener on the supportive or preventive hormones (leptin, ghrelin, adiponectin, insulin) of obesity in the ratsfed the diet containing hydrogenated-vegetable oil and cholesterol. For this purpose, thirtyfemale 5-6 months-old Sprague-Dawley rats were randomly assigned to three groups. Each group consisted of 10 rats. Group 1 (as control group) was fed with a basal diet while the diets of Groups 2 and 3 contained the hydrogenated-vegetable oil (20%) and cholesterol (1%) beside of other nutrients. Garcinia cambogia extract containing 6% hydroxycitric acid was added to the diet w/w of Group 3 after Day 30 up until Day 60. Blood samples were taken from animals on Days 0, 30 and 60 of the trial period. Body weights were weighed in the blood sampling days before sampling. Serum leptin, ghrelin, adiponectin, insulin levels and body weights were not significantly different between groups in the each sampling day. Serum leptin levels were significantly higher on Day 60 than on Days 0 and 30 in Groups 2 and 3. Serum ghrelin levels in Group 3 significantly decreased on Day 30 and significantly increased on Day 60. Serum adiponectin levels in Group 1 were significantly lower on Day 0 than on other days. The levels in Group 3 were significantly higher on Day 60 than on Day 0. The serum insulin levels and body weights were not significantly different between the days in each group. In conclusion, the high lipid diet in doses used in the study (20% hydrogenated-vegetable oil and 1% cholesterol) did not create fat accumulation or obesity in Sprague Dawley rats. Addition of Garcinia cambogia to diet (6%, w/w) of rats fed with the high lipid diet indicated no effect on weight gain. The evidences obtained from this research do not support the role of Garcinia cambogia plant spreadly used as a weight loss facilitator.