Isolation and preparation of monoclonal antibody to ovine Immunoglobulin M

Yilmaz H., Tantas A., Ilgaz A., Morgan K.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, vol.23, no.2, pp.135-140, 1999 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 2
  • Publication Date: 1999
  • Journal Name: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.135-140
  • Istanbul University Affiliated: No

Abstract

Ovine immunoglobulin M was isolated from sheep serum using gel filtration and affinity chromatography. Polyclonal and monoclonal antibodies against this isotype were prepared. The specificity of the monoclonal antibody was investigated using SDS-Page, immunoblotting, an enzyme-linked immunosorbent assay (ELISA) and affinity chromatography. On immunoblotting, monoclonal anti-IgM reacted with a 70 kD protein which corresponded to ovine IgM.