The Frequency of XRCC1 DNA Repair Gene A399G Polymorphism In The Western Anatolia

Sever T., PEHLİVAN S.

EUROPEAN JOURNAL OF THERAPEUTICS, vol.13, no.1, pp.22-25, 2007 (ESCI) identifier

  • Publication Type: Article / Article
  • Volume: 13 Issue: 1
  • Publication Date: 2007
  • Journal Name: EUROPEAN JOURNAL OF THERAPEUTICS
  • Journal Indexes: Emerging Sources Citation Index (ESCI), TR DİZİN (ULAKBİM)
  • Page Numbers: pp.22-25
  • Keywords: XRCC1, RFLP, PCR, DNA, HUMAN
  • Istanbul University Affiliated: Yes

Abstract

The aim of this study is to determine the frequency of polymorphism in codon 399 region of XRCC1 gene in the healthy Western Anatolian population. XRCC1-399 polymorphism is studied in the control group of 100 people living in Izmir, Turkey, who don't have a cancer story, using the method of PCR-RFLP (Msp I Restriction Endonuclease enzyme). The followings are found out as a result of the analyses: the frequency of the ones having A/A genotype is 44.0 %, the frequency of the ones having A/G genotype is 41.0 % and the frequency of the ones having GIG genotype is 15.0 %. Allel frequencies are found to be 64.5 % for A nucleotide and 35.5 % fOr G nucleotide. When the results obtained are compared with various populations, the homozygosity rate for Allel A and Allel G is determined to be at a similar value as the Indian, American, English, Korean and Chinese populations.