Full automation of Ga-68 labelling of DOTA-peptides including cation exchange prepurification


Ocak M. , Antretter M., Knopp R., Kunkel F., Petrik M., Bergisadi N., ...Daha Fazla

APPLIED RADIATION AND ISOTOPES, cilt.68, ss.297-302, 2010 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 68 Konu: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.apradiso.2009.10.006
  • Dergi Adı: APPLIED RADIATION AND ISOTOPES
  • Sayfa Sayıları: ss.297-302

Özet

Here we describe a fully automated approach for the synthesis of Ga-68-labelled DOTA-peptides based on pre-concentration and purification of the generator eluate by using a cation exchange-cartridge and its comparison with fully automated direct labelling applying fractionated elution. Pre-concentration of the eluate on a cation exchange cartridge both using a resin-based and a disposable cation-exchange cartridge efficiently removed Ge-68 as well as major metal contaminations with Fe and Zn. This resulted in a high labelling efficiency of DOTA-peptides at high specific activity (SA) with short synthesis times. (C) 2009 Elsevier Ltd. All rights reserved.