Full automation of Ga-68 labelling of DOTA-peptides including cation exchange prepurification

Ocak M., Antretter M., Knopp R., Kunkel F., Petrik M., Bergisadi N., ...More

APPLIED RADIATION AND ISOTOPES, vol.68, no.2, pp.297-302, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 68 Issue: 2
  • Publication Date: 2010
  • Doi Number: 10.1016/j.apradiso.2009.10.006
  • Journal Name: APPLIED RADIATION AND ISOTOPES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.297-302
  • Keywords: Ge-68/Ga-68 generators, Ga-68-DOTATOC, Automated synthesis, Radiolabelled peptides, Cation exchange, GENERATOR SYSTEM, PET
  • Istanbul University Affiliated: Yes

Abstract

Here we describe a fully automated approach for the synthesis of Ga-68-labelled DOTA-peptides based on pre-concentration and purification of the generator eluate by using a cation exchange-cartridge and its comparison with fully automated direct labelling applying fractionated elution. Pre-concentration of the eluate on a cation exchange cartridge both using a resin-based and a disposable cation-exchange cartridge efficiently removed Ge-68 as well as major metal contaminations with Fe and Zn. This resulted in a high labelling efficiency of DOTA-peptides at high specific activity (SA) with short synthesis times. (C) 2009 Elsevier Ltd. All rights reserved.