Full automation of Ga-68 labelling of DOTA-peptides including cation exchange prepurification

Ocak M., Antretter M., Knopp R., Kunkel F., Petrik M., Bergisadi N., ...Daha Fazla

APPLIED RADIATION AND ISOTOPES, cilt.68, sa.2, ss.297-302, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 68 Sayı: 2
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.apradiso.2009.10.006
  • Dergi Adı: APPLIED RADIATION AND ISOTOPES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.297-302
  • Anahtar Kelimeler: Ge-68/Ga-68 generators, Ga-68-DOTATOC, Automated synthesis, Radiolabelled peptides, Cation exchange, GENERATOR SYSTEM, PET
  • İstanbul Üniversitesi Adresli: Evet

Özet

Here we describe a fully automated approach for the synthesis of Ga-68-labelled DOTA-peptides based on pre-concentration and purification of the generator eluate by using a cation exchange-cartridge and its comparison with fully automated direct labelling applying fractionated elution. Pre-concentration of the eluate on a cation exchange cartridge both using a resin-based and a disposable cation-exchange cartridge efficiently removed Ge-68 as well as major metal contaminations with Fe and Zn. This resulted in a high labelling efficiency of DOTA-peptides at high specific activity (SA) with short synthesis times. (C) 2009 Elsevier Ltd. All rights reserved.