High Performance Liquid Chromatographic Method for the Determination of Norfloxacin in Pharmaceutical Preparation After Pre-column Derivatization

Ulu S. T.

REVISTA DE CHIMIE, cilt.63, sa.2, ss.127-130, 2012 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 63 Sayı: 2
  • Basım Tarihi: 2012
  • Dergi Adı: REVISTA DE CHIMIE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.127-130
  • İstanbul Üniversitesi Adresli: Hayır

Özet

A sensitive high performance liquid chromatography (HPLC) method for the determination of Nor floxacin in pharmaceutical preparation is described. The proposed method was based on the derivatization of Norfloxacin with 4-chloro-7-nitrobenzofurazan in borate buffer at pH 9.0 to yield a fluorescent product. The chromatographic separation was achieved on a C-18 column (150 mmx4.6 mm) using a mobile phase of methanol-water (75:25, v/v) solvent system at 1.1 mL/min flow-rate. The assay was linear over the concentration range of 10 to 500 ng/mL.