Interciencia, vol.46, no.1, pp.170-177, 2021 (SCI-Expanded)
Objective: In this research, it was aimed to evaluate the effects of
melatonin and vitamin D on zoledronic acid treated human osteoblasts’ vitality.
Our main purpose in this study is try to find an answer to “Can supplement usage
such as melatonin and vitamin D reduce the severity of MRONJ?”
Methods: Human osteoblasts incubated various zoledronic acid
concentrations (10 and 50 μM). Two different concentrations of vitamin D (10
and 100 μM) and melatonin (40 μM) were added in several combinations for 24th
and 48th hour and 36 groups were created. After that cells are collected and the
effects are tested with the use of the proliferation assay (MTT). Data were
collected and analyzed statistically.
Results: There were statistically significant differences between 24th and
48th hour’s results.
Conclusion: Generally, after 24th hour melatonin and vitamin D addition
to zoledronic acid treated human osteoblast can contribute to reset negative effects
of zoledronic acid. Further investigations are needed to suggest melatonin and
vitamin d supplements to MRONJ patients both in vivo and in vitro.