Evaluation of Melatonin and Vitamin D’s Effects on Zoledronic Acid Treated Osteoblasts’ Vitality, in Vitro

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Koltuk M., Karaçam B., Çakiris A., Abacı N., Sırma Ekmekci S., Gürkan Köseoğlu B.

Interciencia, vol.46, no.1, pp.170-177, 2021 (SCI-Expanded)

  • Publication Type: Article / Article
  • Volume: 46 Issue: 1
  • Publication Date: 2021
  • Journal Name: Interciencia
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Agricultural & Environmental Science Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Veterinary Science Database, DIALNET
  • Page Numbers: pp.170-177
  • Istanbul University Affiliated: Yes


Objective: In this research, it was aimed to evaluate the effects of

melatonin and vitamin D on zoledronic acid treated human osteoblasts’ vitality.

Our main purpose in this study is try to find an answer to “Can supplement usage

such as melatonin and vitamin D reduce the severity of MRONJ?”

Methods: Human osteoblasts incubated various zoledronic acid

concentrations (10 and 50 μM). Two different concentrations of vitamin D (10

and 100 μM) and melatonin (40 μM) were added in several combinations for 24th

and 48th hour and 36 groups were created. After that cells are collected and the

effects are tested with the use of the proliferation assay (MTT). Data were

collected and analyzed statistically.

Results: There were statistically significant differences between 24th and

48th hour’s results.

Conclusion: Generally, after 24th hour melatonin and vitamin D addition

to zoledronic acid treated human osteoblast can contribute to reset negative effects

of zoledronic acid. Further investigations are needed to suggest melatonin and

vitamin d supplements to MRONJ patients both in vivo and in vitro.