Selective and oxidative stress-mediated cell death of MCF-7 cell line induced by terpinolene


Kig C., Mertoglu E., Caliskan A., Ağuş H. H. , Ucar E. O. , Guler V.

BIOLOGIA, 2021 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume:
  • Publication Date: 2021
  • Doi Number: 10.1007/s11756-021-00803-z
  • Journal Name: BIOLOGIA
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, Animal Behavior Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Veterinary Science Database
  • Keywords: Terpinolene, MCF7, HEK293, apoptosis, necrosis, oxidative stress, CHEMICAL-COMPOSITION, NATURAL-PRODUCTS, SIGNALING PATHWAYS, ESSENTIAL OIL, CYTOTOXICITY, APOPTOSIS, ANTICANCER, TERPINEOL, EXPOSURE, GROWTH

Abstract

In this study, we compared the effects of terpinolene against the anti-cancer agent cisplatin on proliferation, apoptotic activity and intracellular ROS (reactive oxygen species) production in cancerous (MCF-7) and non-cancerous (HEK-293) cell lines. Cisplatin presented a strong growth-inhibitory effect on both MCF-7 (IC25: 18.77 mu M) and HEK-293 (IC25: 15.03 mu M) cell lines. The growth-inhibitory effect of terpinolene was found to be weaker than cisplatin (IC25: 291.18 mu M for MCF-7 and 345.35 mu M for HEK 293 cells). The expression levels of BAX (by 1.86-fold), cleaved-PARP (by 2.23-fold) and pro-caspase-8 (by 1.74-fold) proteins increased in response to terpinolene treatment in MCF-7 cells. Curiously, the increase in the expression levels of these apoptotic markers was less pronounced in terpinolene-treated non-cancerous HEK 293 cells. Terpinolene, promoted a higher rate of apoptotic and necrotic cell death on MCF-7 cells (apoptotic cells: 25.28 %; necrotic cells: 17.70 %) when compared with HEK-293 cell line (apoptotic cells: 15.47 %; necrotic cells: 9.42 %). Interestingly, terpinolene caused a marked increase (2.05-fold) in intracellular ROS production in MCF-7 cells while HEK-293 cells appeared to be resistant to terpinolene or cisplatin treatments. In conclusion, although terpinolene showed a weaker growth-inhibitory effect on MCF-7 cancer cells, it exhibited a better selectivity than cisplatin in inducing cell death and intracellular oxidative stress in MCF7 breast cancer cells.