EFFECTS OF DIFFERENT DOSES OF TAURINE IN THE GLUCOSE-BASED EXTENDER DURING CRYOPRESERVATION OF RAINBOW TROUT (<i>ONCORHYNCHUS MYKISS</i>) SEMEN

Ekici A., Baran A., Yamaner G., Ozdas O. B., Sandal A. I., Guven E., ...More

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, no.4, pp.3113-3115, 2012 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Publication Date: 2012
  • Doi Number: 10.5504/bbeq.2012.0041
  • Journal Name: BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.3113-3115
  • Keywords: Rainbow trout, Onchorhynchus mykiss, taurine, sperm, cryopreservation, LIPID-PEROXIDATION, SUPEROXIDE-DISMUTASE, OXIDATIVE STRESS, SPERM, ANTIOXIDANTS, HYPOTAURINE, SPERMATOZOA, FERTILITY, MOTILITY, PARAMETERS
  • Istanbul University Affiliated: Yes

Abstract

This study aimed to investigate the effects of different doses of taurine added to glucose extender Semen was collected from rainbow trout (Oncorhynchus mykiss) by abdominal massage and diluted (1:2) with 300 mM glucose (G) extender containing different taurine doses (50, 75 and 100 mM). The control group extender did not contain taurine. After dilution and dosing, samples were filled to 0.5 ml straws, frozen in nitrogen vapour and stored in liquid nitrogen. The comparison of the results obtained for the 50 mM taurine group and the control group showed no significant difference between the post-thawing motility percentages and motility duration of sperm, and only a very low statistically difference for eyed-embryo percentages (P < 0.05).