Akademik Veri Yönetim Sistemi
Kafkas Üniv. Vet. Fak. Derg., cilt.14, sa.2, ss.157-160, 2008 (SCI-Expanded)
Bu calşmada, embriyonik gelişim srasnda uygulanan genisteinin civciv testisleri uzerinde gostereceği
etkilerin belirlenmesi amacland. İnkubasyonun 4. gununde, soya fasulyesinden elde edilmiş genistein,
yumurtann kut ucundan kucuk bir delik aclarak yumurta sarsna enjekte edildi. Ckmdan 3 gun sonra
erkek ciccivlerin testisleri alnarak paraffin kesitleri hazrland. Standart nokta saym yontemiyle Sertoli
hucreleri ve germ hucrelerinin nukleus hacim yoğunluklar ve toplam hacimleri belirlendi. Calşma
sonucunda embriyonik donemde uygulanan genisteinin, civciv testisleri uzerinde olumsuz bir etki
gostermediği saptand. Aksine germ hucrelerinin gelişimini olumlu yonde etkilediği belirlendi.
There is no in vivo study on the effect of phytoestrogens on the development of testis in domestic fowl;
therefore the aim of this study was to examine the effect of genistein on the cockerel testis during embryonic
development. Genistein from soybean were injected into yolk via a small hole at the blunt end of the egg on
day 4 of incubation. Cockerels were decapitated on post-hatching day 3 and paraffin sections of the testes
were prepared. Nuclear volume density and absolute volume of Sertoli cells and germ cells, seminiferous
tubule volume density and absolute volume were determined using the standard point counting method.
Genistein did not cause any inhibitory effect on the embryonic development of cockerel testis. On the
contrary it had a stimulatory effect on germ cell development.