iCELLigence real-time cell analysis system for examining the cytotoxicity of drugs to cancer cell lines.

Sener L., Albeniz G., Dinc B., Albeniz I.

Experimental and therapeutic medicine, cilt.14, sa.3, ss.1866-1870, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 3
  • Basım Tarihi: 2017
  • Doi Numarası: 10.3892/etm.2017.4781
  • Dergi Adı: Experimental and therapeutic medicine
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1866-1870
  • İstanbul Üniversitesi Adresli: Evet

Özet

The recently developed iCELLigence (TM) real-time cell analyzer (RTCA) can be used for the label-free real-time monitoring of cancer cell proliferation, viability, invasion and cytotoxicity. The RTCA system uses 16-well microtiter plates with a gold microelectrode biosensor array that measures impedance when cells adhere to the microelectrodes causing an alternating current. By measuring the electric field generated in this process, the RTCA system can be used for the analysis of cell proliferation, viability, morphology and migration. The present review aimed to summarize the working method of the RTCA system, in addition to discussing the research performed using the system for various applications, including cancer drug discovery via measuring cytotoxicity.