Akademik Veri Yönetim Sistemi
International conference on nutritional strategies and food science innovations (ICNSFSI - 26), Manila, Filipinler, 20 - 21 Mart 2026, ss.10-11, (Özet Bildiri)
This study presents the valorization of Rapana venosa, an ecologically invasive marine gastropod in the Black Sea, as a novel source of protein for the development of nanomaterials. Firstly, Rapa whelks collected from different stations of Black Sea coast and then homoganetes prepared. Homogenate powder contained 6.54±0.07% moisture, 13.45±0.58% lipid 7.22±0.94% ash, and 15.02±1.83% carbohydrate. A nitrogen solubility profile was generated to identify the optimal pH for protein extraction, showing minimal solubility at pH 5.0 (isoelectric point). pH 9.0 was selected as the maximum solubility value for industrial relevance due to its lower chemical usage and cost efficiency. Protein concentrates and hydrolysates were extracted using pH-shift precipitation and enzymatic hydrolysis with Alkalase, respectively. Protein content (from 57.48±1.57 to 65.37±0.25), total nitrogen (from 10.70±0.29 to 12.51±0.03, protein nitrogen (from 7.54±0.09 to 12.17±0.05) values were increased for concentrates compared to homogenates (p<0.05). Degree of hydrolysis and amino nitrogen contents in concentrates were determined in 0, 30, 60,120 and 240 min incubatation intervals. Alcalase hydrolysis of proteins, as visualized by SDS–PAGE, progressively degraded high molecular weight bands into low molecular weight peptides confirming structural breakdown over time. FTIR confirmed slight shifts in the bands of the peptide chain (in the skeleton amino acids), carboxyl groups (H-C-O-O), O-H bending bands and CH2 stretching bands overlapping with C-H stretching vibrations at different intervals. Twenty amino acid composition and total amino acid contents were found as 736.53±0.65 mg/L for concentrate and 813.02±0.52, 848.64±0.58, 752.12±0.63 mg/L after 30-60-120 240 min alcalase treatments, respectively. ABTS (9.06) and DPPH assays (9.21) in selected 60 min treated hydrolysate indicated antioxidant activity. SEM revealed differences in morphology between concentrate and hydrolysates, highlighting the influence of peptide chain length and solubility. Collectively, the findings suggest that Rapana venosa protein-derived hydrolysates are promising to fabricate nanomaterials as bioactive films for seafood packaging. Results introduces novel marine biopolymer source, contributing to the sustainable management of an ecologically disruptive species
. Keywords: Rapana venosa, aquatic protein, amino acid profile, enzymatic hydrolysate.
Acknowledgments: This study was funded by Istanbul University (Research Universities Support Program) under Grant Agreement No FBA-2025-4134