Plasmid-Mediated Quinolone Resistance Genes in Escherichia coli Urinary Isolates from Two Teaching Hospitals in Turkey: Coexistence of TEM, SHV, CTX-M and VEB-1 Type beta-lactamases


Nazik H., BEKTORE B., Ongen B. , Ilktac M., OZYURT M., Kuvat N., ...Daha Fazla

TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH, cilt.10, ss.325-333, 2011 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 10 Konu: 3
  • Basım Tarihi: 2011
  • Doi Numarası: 10.4314/tjpr.v10i3.9
  • Dergi Adı: TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
  • Sayfa Sayıları: ss.325-333

Özet

Purpose: To evaluate the occurrence of plasmid-mediated quinolone resistance (PMQR) genes and the prevalence of extended spectrum beta-lactamase (ESBL) types in Escherichia coli clinical isolates.

Plasmid-Mediated Quinolone Resistance Genes in Escherichia coli Urinary Isolates from Two Teaching Hospitals in Turkey: Coexistence of TEM, SHV, CTX-M and VEB-1 Type beta-lactamases

By:Nazik, H (Nazik, Hasan)1 ] Bektore, B (Bektore, Bayhan)2 ] Ongen, B (Ongen, Betigul)1 ] Ilktac, M (Ilktac, Mehmet)1 ] Ozyurt, M (Ozyurt, Mustafa)2 ] Kuvat, N (Kuvat, Nuray)1 ] Baylan, O (Baylan, Orhan)2 ] Kekulluoglu, H(Kekulluoglu, Hatun)1 ] Haznedaroglu, T (Haznedaroglu, Tuncer)2 ] Kelesoglu, FM (Kelesoglu, Fatih Mehmet)3 ]

 

TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH

 

Volume: 10

 

Issue: 3

 

Pages: 325-333

DOI: 10.4314/tjpr.v10i3.9

Published: JUN 2011

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Abstract

Purpose: To evaluate the occurrence of plasmid-mediated quinolone resistance (PMQR) genes and the prevalence of extended spectrum beta-lactamase (ESBL) types in Escherichia coli clinical isolates. 

Methods: Sixty-one ESBL-producing urinary E. coli isolates were studied. An antibiotic susceptibility test was performed using the disc diffusion method. ESBL production was determined using a double-disc synergy test for all isolates; E-test and Vitek 2 were used for plasmid-mediated quinolone resistance (PMQR)-positive isolates and their transconjugants. The presence of PMQR and beta-lactamase genes was determined by polymerase chain reaction (PCR). 

Results: The strains displayed high rates of resistance to norfloxacin (80 %). The most frequent PMQR gene was aac(6')-Ib-cr (45.9 %). In all, one qnrA1 (1.6 %), one qnrS1 (1.6 %), and two qepA1-positive isolates (5.7 %) were identified. The genes, qnrS1+aac(6')-Ib-cr and qepA1, were co-expressed with bla(CTX-M-15) gene, while qnrA1 occurred with bla(TEM-1), bla(SHV), and bla(VEB-1) genes. The most frequent beta-lactamase type was cefotaximase (CTX-M), which generally hydrolyzes cefotaxime (92 %) more than it does ceftazidime; followed by temoneira (TEM, 39 %); sulfhydryl variable (SHV, 5 %), and Vietnamese extended-spectrum beta-lactamase (VEB, 1.6 %). 

Conclusion: A high prevalence of aac(6')-Ib-cr and CTX-M type beta-lactamase was detected in ESBL-producing E. coli strains. This study also identified the co-expression of qnrA1 and blaVEB-1 genes and of qnrS1+aac(6')-Ib-cr in E. coli isolates. The co-existence of PMQR genes with ESBLs may lead to a serious public health problem.