Akademik Veri Yönetim Sistemi
TuPA International Proteomics Congress 7 Turkish National Proteomics Congress, İstanbul, Türkiye, 18 - 19 Eylül 2025, ss.13, (Özet Bildiri)
Aim: This study aimed to implement the first palaeoproteomic application at a university centre in Türkiye. To this end, Zooarchaeology by Mass Spectrometry (ZooMS) approach was applied to five archaeological bone finds dated to the Hellenistic period from the Antandros Ancient City excavations (Balıkesir, Türkiye). Methods: Bone samples were collected under sterile conditions during the 2024 excavations at Antandros. In addition, bone grave gifts from earlier seasons were delivered to our laboratory. Exterior decontamination was performed with acetone and distilled water. The decontaminated samples were then powdered using a Dremel tool. Type I collagen was obtained by HCl demineralisation procedure followed by gelatinisation in ammonium bicarbonate buffer (AmBic). Following isolation, A205/A280 spectrophotometric readings and the BCA assay provided a preliminary assessment of collagen quantity. The five samples were then submitted for MALDI-TOF MS analysis at Acıbadem University following trypsin digestion. The resulting mass spectras were analysed with the semi-automated SpecieScan tool. Results: MALDI spectra were obtained from all five samples; in 4/5 specimens, candidate higher-taxon/species groups passed the correlation threshold (mostly terrestrial mammals). In 1/5 specimens, peptide motifs indicating a marine mammal were observed despite being below the threshold. Although collagen preservation varied among samples, the workflow produced results quickly and showed consistent similarity values across technical replicates. Conclusion: This pilot demonstrates that a ZooMS-based palaeoproteomic application and palaeoproteomics research environment can be established in Türkiye. The findings suggest potential raw-material diversity in Hellenistic bone objects; however, low-correlation signals will undergo additional validation. Peptide signals from a bone hairpin deposited as a grave good that indicate a marine mammal suggest that, as today, bones from particular species may also have been valued in that period. With a larger sample set curated by our ongoing project, the importance of palaeoproteomics for archaeology will be further highlighted. Keywords: Palaeoproteomics, Peptide Mass Fingerprinting, Zooarchaeology by Mass Spectrometry