Atıf İçin Kopyala
Cihanoglu N., Adaleti R., Nakipoglu Y.
CLINICAL LABORATORY, cilt.65, ss.93-99, 2019 (SCI-Expanded)
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Yayın Türü:
Makale / Tam Makale
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Cilt numarası:
65
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Basım Tarihi:
2019
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Doi Numarası:
10.7754/clin.lab.2018.180625
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Dergi Adı:
CLINICAL LABORATORY
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Derginin Tarandığı İndeksler:
Science Citation Index Expanded (SCI-EXPANDED), Scopus
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Sayfa Sayıları:
ss.93-99
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İstanbul Üniversitesi Adresli:
Evet
Özet
Background: We aimed to investigate the frequency of fibronectin binding protein (FBP), which is part of the first
step of adhesion, and Panton-Valentine leukocidin (PVL) toxin, which contributes to the destruction of host leukocytes
and tissue necrosis, in clinical S. aureus strains.
Methods: One hundred S. aureus strains were included in the study and distributed as follows; 33 from skinwound
swabs and catheter tips (SWCT), 33 from body fluid and secretion specimens (BSFS) such as tracheal aspirate,
sputum, and pleural effusion fluid, 18 from tissue biopsy specimens (TBS), 10 specimens from blood, and related
specimens (BRS) such as bone marrow, and cerebral spinal fluid, and six specimens from mucosal membrane
of pharynx, nose, and vagina (MMS). Methicillin resistance was tested by disk diffusion method. mecA
(methicillin resistance coded gene), pvl and fnbA genes were investigated by using a PCR method.
Results: Thirty-seven strains (37.0%) were identified as methicillin resistant S. aureus (MRSA) and 63 (63.0%) as
methicillin susceptible S. aureus (MSSA) strains. fnbA was more frequent in S. aureus isolates of MMSs (100.0%);
followed by BRSs (80.0%), SWCTs (78.8%), TBS (72.3%), and BSFs (66.7%), whereas pvl gene was more frequent
in isolates of BRS (60.0%), followed by TBSs (50.0%), SWCTs (33.4%), BSFs (30.3%), and MMSs (16.7%).
fnbA existed in 85.7% of MSSA and 56.8% of MRSA in contrast to pvl, which was more frequent in MRSA
(70.3%) than those of MSSA strains (17.4%). These differences were statistically significant (p < 0.05).
Conclusions: Our different clinical specimens contained a high rate of fnbA (75.0%) and low-moderate frequency
of pvl (37.0%). fnbA was most frequent in S. aureus of MMSs, followed by BRSs, and SWCTs, whereas pvl was existed
in high proportion in S. aureus of BRSs, followed by TBSs, and SWCTs. Presence of PVL in a high proportion
in MRSA strains of superfical specimens such SWCT (24.4%) and deeper serious specimens such as BRS
(16.3%) compared to MSSA strains from the same specimens, 3.2% and 0%, respectively, have shown that MRSA
infections still threatens patients’ lives and control of their spread is urgently needed.
(Clin. Lab. 2019;65:93-99. DOI: 10.7754/Clin.Lab.2018.180625)