Cereal Research Communications, vol.0, no.0, 2021 (SCI-Expanded)
Soil salinity is one of the
most significant environmental constraints to global agricultural productivity.
To meet the nutritional needs of the growing population, cereals such as wheat,
which are essential for dietary products but also sensitive to salinity, must
be improved to be either more resilient or less susceptible to salt stress. The
main objective of this study was to examine the responses of advanced mutant
lines to salt stress. Five-advanced mutant wheat lines, which were previously
found to be drought tolerant, were grown under 150 mM NaCl stress conditions to
see how they responded to salt stress conditions. They were generated via 200
Gy gamma-ray applications to the Sagittario commercial cultivar. The results
show that the content of thiobarbituric acid-reactive substances (TBARS),
chlorophyll, and electrolyte
leakage % did not change significantly. Mutant lines showed higher antioxidant
defense system parameters (SOD, CAT, POX, and GR). Similarly, the amount of
proline involved in intracellular homeostasis was higher in all mutants. Under
saline stress, the expression of Triticum aestivum Salt Overly Sensitive 1 (TaSOS1)
increased in all mutant lines, the expression of Triticum aestivum
High-Affinity Potassium ion Transporter 2;1 (TaHKT2;1) increased in all except in mutant line 5, and the
expression of Triticum aestivum Sodium ion / Hydrogen ion vacuolar
antiporter (TaNa+/H+
vacuolar antiporter) increased in all except in mutant line 3. Synergetic
interaction in the expression of the genes involved in signal transduction
resulted in more intracellular K+. Overall, these results suggest
that mutants’ responses to salinity stress is related to an ability to
accumulate K+ in cells with synergetic expressional regulation of TaHKT2;1, TaNa+/H+ vacuolar antiporter, and TaSOS1 genes, in addition to responses
of antioxidant enzyme activities and the accumulation of proline.
Soil salinity is one of the
most significant environmental constraints to global agricultural productivity.
To meet the nutritional needs of the growing population, cereals such as wheat,
which are essential for dietary products but also sensitive to salinity, must
be improved to be either more resilient or less susceptible to salt stress. The
main objective of this study was to examine the responses of advanced mutant
lines to salt stress. Five-advanced mutant wheat lines, which were previously
found to be drought tolerant, were grown under 150 mM NaCl stress conditions to
see how they responded to salt stress conditions. They were generated via 200
Gy gamma-ray applications to the Sagittario commercial cultivar. The results
show that the content of thiobarbituric acid-reactive substances (TBARS),
chlorophyll, and electrolyte
leakage % did not change significantly. Mutant lines showed higher antioxidant
defense system parameters (SOD, CAT, POX, and GR). Similarly, the amount of
proline involved in intracellular homeostasis was higher in all mutants. Under
saline stress, the expression of Triticum aestivum Salt Overly Sensitive 1 (TaSOS1)
increased in all mutant lines, the expression of Triticum aestivum
High-Affinity Potassium ion Transporter 2;1 (TaHKT2;1) increased in all except in mutant line 5, and the
expression of Triticum aestivum Sodium ion / Hydrogen ion vacuolar
antiporter (TaNa+/H+
vacuolar antiporter) increased in all except in mutant line 3. Synergetic
interaction in the expression of the genes involved in signal transduction
resulted in more intracellular K+. Overall, these results suggest
that mutants’ responses to salinity stress is related to an ability to
accumulate K+ in cells with synergetic expressional regulation of TaHKT2;1, TaNa+/H+ vacuolar antiporter, and TaSOS1 genes, in addition to responses
of antioxidant enzyme activities and the accumulation of proline.