LC determination of amino glutethimide enantiomers as dansyl and fluorescamine derivatives in tablet formulations


Cesur N., Apak T., Aboul-Enein H., Ozkirimli S.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, vol.28, pp.487-492, 2002 (SCI-Expanded) identifier identifier identifier

Abstract

Determination of dansyl (AG-DNS) and fluorescamine (AG-F) derivatives of rac-aminoglutethimide in tablet formulation by HPLC has been achieved on a cellulose tris-(3,5-dimethylphenyl carbamate), known as Chiralcel OD and OD-R under normal and reversed phase columns, respectively, using a fluorescence detector (lambda(ex), 360 nm; lambda(em), 530 nm for AG-DNS derivatives; lambda(ex), 395 nm; lambda(em), 495 nm for fluorescamine derivatives (AG-F)). The best results were obtained with mobile phase ethanol:cyclohexane:methanol (95:5:2 v/v/v) for AG-DNS derivatives and acetonitrile:0.5% ortho-phosphoric acid (85:15 v/v) containing 0.26 mM 1-hexanesulfonic acid sodium salt (HSA) for AG-F, respectively. The lower limit of detection (signal to noise ratio of 3:1) were found to be 20 ng ml(-1) for each enantiomer for AG-DNS and 20.5 ng ml(-1) for each diastreoisomer for AG-F. (C) 2002 Elsevier Science B.V. All rights reserved.