Simultaneous determination of desloratadine and pseudoephedrine sulfate in tablets by high performance liquid chromatography and derivative spectrophotometry


Caglar S. , Toker S.

REVIEWS IN ANALYTICAL CHEMISTRY, cilt.30, ss.145-151, 2011 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 30
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1515/revac.2011.100
  • Dergi Adı: REVIEWS IN ANALYTICAL CHEMISTRY
  • Sayfa Sayıları: ss.145-151

Özet

Simple and accurate reversed phase liquid chromatographic and first derivative spectrophotometric methods have been developed for simultaneous determination of desloratadine and pseudoephedrine in combined dosage forms. The first method is based on the separation of both drugs by high performance liquid chromatography using a C18 column and 10 m m orthophosphoric acid: acetonitrile (77: 23). The drugs are eluted at 1.0 ml/min flow rate and detected at 262 nm. Ondansetron was used as internal standard. The calibration graphs of desloratadine and pseudoephedrine were linear in the range of 1.0-10.0 mu g/ml and 48-480 mu g/ml, respectively. The detection limits of the method were found to be 0.14 mu g/ml and 3.80 mu g/ml for desloratadine and pseudoephedrine, respectively. The derivative spectrophotometric method depends on measuring the first derivative amplitudes at 280 and 244 nm for desloratadine and pseudoephedrine, respectively. The calibration graphs were linear in the range of 2.5-15.0 mu g/ml for desloratadine and 120-720 mu/ml for pseudoephedrine. The detection limits were found at 0.38 mu g/ml and 21.45 mu g/ml for desloratadine and pseudoephedrine, respectively. The proposed methods were successfully applied to the determination of these drugs in commercially available tablets.
Simultaneous determination of desloratadine and pseudoephedrine sulfate in tablets by high performance liquid chromatography and derivative spectrophotometry
Author(s): Caglar, S (Caglar, Sena)1 ] Toker, SE (Toker, Sidika Erturk)1 ]
Source: REVIEWS IN ANALYTICAL CHEMISTRY  Volume: 30   Issue: 3-4   Pages: 145-151   DOI:10.1515/REVAC.2011.100   Published: NOV 2011
Times Cited: 2 (from Web of Science)
Cited References: 27      [ view related records ]     Citation MapCitation Map     
Abstract: Simple and accurate reversed phase liquid chromatographic and first derivative spectrophotometric methods have been developed for simultaneous determination of desloratadine and pseudoephedrine in combined dosage forms. The first method is based on the separation of both drugs by high performance liquid chromatography using a C18 column and 10 m m orthophosphoric acid: acetonitrile (77: 23). The drugs are eluted at 1.0 ml/min flow rate and detected at 262 nm. Ondansetron was used as internal standard. The calibration graphs of desloratadine and pseudoephedrine were linear in the range of 1.0-10.0 mu g/ml and 48-480 mu g/ml, respectively. The detection limits of the method were found to be 0.14 mu g/ml and 3.80 mu g/ml for desloratadine and pseudoephedrine, respectively. The derivative spectrophotometric method depends on measuring the first derivative amplitudes at 280 and 244 nm for desloratadine and pseudoephedrine, respectively. The calibration graphs were linear in the range of 2.5-15.0 mu g/ml for desloratadine and 120-720 mu/ml for pseudoephedrine. The detection limits were found at 0.38 mu g/ml and 21.45 mu g/ml for desloratadine and pseudoephedrine, respectively. The proposed methods were successfully applied to the determination of these drugs in commercially available tablets.
Accession Number: WOS:000297461600003
Document Type: Article