Method development and validation of a rapid determination of ropinirole in tablets by LC-UV


Oenal A.

CHROMATOGRAPHIA, vol.64, pp.459-461, 2006 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 64
  • Publication Date: 2006
  • Doi Number: 10.1365/s10337-006-0045-1
  • Journal Name: CHROMATOGRAPHIA
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.459-461

Abstract

A reversed-phase high-performance liquid chromatographic (HPLC) method with UV detection was developed and validated for the determination of ropinirole (ROP) in tablets. The assay utilized UV detection at 250 nm and a Luna CN column (250 x 4.6 mm I.D, 5 mu m). The mobile phases were comprised of acetonitrile: 10 mM nitric acid (pH 3.0) (75:25, v/v). Validation experiments were performed to demonstrate linearity, accuracy, precision, limit of quantitation (LOQ), limit of detection (LOD), and robustness. The method was linear over the concentration range of 0.5-10.0 mu g mL(-1). The method showed good recoveries (99.75-100.20%) and the relative standard deviations of intra and inter-day assays were 0.38-1.69 and 0.45-1.95%, respectively. The method can be used for quality control assay of ropinirole.