The End of a Mythical Chromosome Story: Karyomorphology of Crocus mathewii Kerndorff &Pasche


Çiftçi A., Şik L., Tuna M., Erol O.

8. Asia-pasific Chromosome Colloqium, Tekirdağ, Türkiye, 17 - 21 Ekim 2023

  • Yayın Türü: Bildiri / Yayınlanmadı
  • Basıldığı Şehir: Tekirdağ
  • Basıldığı Ülke: Türkiye
  • İstanbul Üniversitesi Adresli: Evet

Özet

Crocus L. taxa in Turkey is known to usually have 2n=6 to 30 chromosomes with the exception of Crocus mathewii, which is known to has 2n=70 chromosomes. However, there is no metaphase plate seen and hence it has been a suspicious record. Crocus mathewii Kerndorff & Pasche (Iridaceae) is a rare and endemic taxon from the Western Taurus region (Turkey) and its habitats are under anthropogenic pressure. Crocus mathewii has two forms; one with a Sulphur throat and other with yellow throat. In some populations both of these forms can be seen. In the current study, karyomorphological analysis of 7 C. mathewii populations are examined. Five to eight corms were collected from each population for this study. The root tips were fixed in Carnoy’s solution and stained with Acetocarmin. At least five monolayer metaphase plates from different individuals were examined from each population. Genome sizes were measured from 3 individuals from each populations and found between 6.13 and 6.79. All individuals from the type locality were diploid and somatic chromosome number were found as 2n=16. The mixed colored populations along with all white populations chromosome numbers were found 2x=2n=14.

This work was supported by Istanbul University Scientific Research Committee through grant no. 36860.

Key words: Chromosome, 2C DNA, cytogenetic, karyotypic variation