Expression patterns and clinical relevance of H19, miR-181a-3p, and TGF-β in ascending aortic aneurysms: a comparative analysis of human aortic tissue

Karpuzoğlu F. H., Çalışkan R., Gümüş Ş. N., Küçükgergin C., Aydın A. F., Karpuzoğlu E., ...Daha Fazla

Molecular Biology Reports, cilt.52, sa.1, 2025 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 52 Sayı: 1
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1007/s11033-025-10740-8
  • Dergi Adı: Molecular Biology Reports
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Veterinary Science Database
  • Anahtar Kelimeler: Ascending aortic aneurysms, LncRNA H19, miR-181a-3p, Non-coding RNAs (ncRNAs), TGF-β
  • İstanbul Üniversitesi Adresli: Evet

Özet

Background: Ascending aortic aneurysms (AsAA) are progressive dilations of the aortic wall associated with pathological remodeling. Non-coding RNAs, including lncRNA H19 and miR-181a-3p, regulate vascular remodeling and inflammation, partly through modulation of the TGF-β signaling pathway. Methods and results: This study compared the expression of H19, miR-181a-3p, and TGF-β in aortic tissues from 48 patients undergoing AsAA or multivessel coronary artery bypass grafting (mCABG). Aortic tissue samples were collected, and RNA was extracted for quantitative real-time PCR analysis. MiR-181a-3p expression was significantly lower in the AsAA group compared to the mCABG group (p = 0.004), while TGF-β expression was higher (p = 0.022). When adjusted for DM, the difference in TGF-β expression became insignificant and H19 expression showed no significant difference between AsAA and mCABG groups. Conclusions: The decreased expression of miR-181a-3p in AsAA suggests its potential as a biomarker. Further studies are needed to explore its role in AsAA pathophysiology.