Akademik Veri Yönetim Sistemi
Turkish & Italian Joint International Animal Reproduction Congress, Antalya, Türkiye, 10 - 13 Ekim 2024, ss.332-335, (Tam Metin Bildiri)
Boron (B) is the 5th element in the periodic table and is found in nature as
borax, boron, boric acid, colemanite, kernite, ulexite, and borate (4). It also
plays a role in the absorption and balance of phosphorus, magnesium, and
calcium in bones and joints (5, 10, 11). Although the biochemical mechanism
of boron is not fully known, it is thought that it may be a metabolic regulator
in cell membrane functions (12) and enzymatic system (5). In addition, it also
has antibacterial and antifungal effects. There are a limited number of studies
in the literature regarding animal embryo culture with boron or boric acid.
The first study on embryo development in the literature found that 12-25 μM
boric acid supported blastocyst development with the addition of mouse
embryo culture (9). There were several studies on animal reproduction of
boron for potential beneficial effects (1, 3, 6-8, 13). In addition, in a recent
study, 20-250 μM/L boric acid as an antioxidant did not support embryo
development after developing in vitro bovine embryo culture conditions (7).
According to the latest research in the literature; after adding 2, 1 and 0.4 μg
of boric acid to the in vitro culture medium for bovine embryo development,
blastocyst development was 47%, 49%, 54%, respectively, and a significant
difference was observed positively compared to the control group. In this
study, the highest improvement was achieved especially in the low dose 0.4
μg boric acid group (13). In our study, boric acid at doses of 1.62x10-1 μM,
1.62x10-3 μM, 1.62x10-5 μM was added to the in vitro maturation medium of
ovine oocytes obtained from the slaughterhouse and incubated for 24 hours
to mature and reach the MI stage.