The Southwestern Black Sea region has an intense city life where swimming, fishery and shipping are widespread practices. It is crucial to determine and trace the levels of microbiological contamination of such places in terms of public health. Within the scope of this study, we collected samples from the surface water of the sea in 10 different areas (St1-10) between Igneada-Kilyos seasonally and analyzed them in terms of fecal coliform (FC), total coliform (TC), fecal streptococci (FS), total mesophilic heterotrophic bacteria (TM), Pseudomonas spp. (P) and Aeromonas spp. (A) through bacterial isolation. The antibiotic and heavy metal resistance profiles of the isolated bacteria were also investigated. According to results, the most polluted stations are St5, St7 and St10. When the results were evaluated on a seasonal basis, the highest TC, TM, P and A counts were observed in the summer while the maximum FC and FS counts were observed in the winter and autumn, respectively. When FC/FC ratios were examined, it was detected that the contamination in St1-3 was of only animal origin, but the contamination in the other stations was of both animal and human origin. In this study, we isolated 153 bacteria of 43 different species, notably E. coli (25%), Fecal streptococci (15%), Pseudomonas fluorescens (9%) and Aeromonas hydrophila (8%). When the antibiotic and heavy metal resistance profiles of the isolated bacteria were examined, it was observed that the stations had been exposed to a serious contamination stemming from human activities in relation to fecal and industrial wastes.