In this study, it was aimed to determine and overcome undergraduates' laboratory anxiety. For this purpose, Laboratory Anxiety Questionnaire (LAQ) was developed by researchers. LAQ was applied to 92 undergraduates as a pre-test and focus group interviews were performed to determine their laboratory anxiety. An action research was conducted by researchers. After instruction was accomplished in ten weeks, LAQ was applied as post-test. According to results, it was found that undergraduates' laboratory anxiety related to working chemicals especially acids, using laboratory materials and equipments, laboratory accidents and making mistake, which were determined in the pre-test, were overcame in highly percentages. (C) 2014 The Authors. Published by Elsevier Ltd.