Small Meeting on Yeast Transport and Energetics, Turkey, 1 - 04 September 2013, pp.24
Glucose is the preferred carbon and energy source in most organisms as well as playing actively a role in the regulation of many biological processes. It has been reported that many studies of calorie restriction arise from glucose depletion increased the life span in Saccharomyces cerevisiae and S.pombe. It was known that the relationship between glucose sensing/signaling and oxidative stress response. In parallel, in our previous study, it was supported these relationship. We aim to reveal the role of glucose transporters on oxidative stress response in S. pombe using glucose repression-resistant mutants of S. pombe (ird5, ird11, ird13 ve ird14) which are developed in laboratories of Istanbul University Molecular Biology and Genetics Department. In this study, the expression levels of glucose transport genes (ght1, ght2, ght3, ght4, ght5, ght6, ght7 and ght8) and some genes involved in glucose sensing (git3 and ssp2) in ird mutants treated with/without hydrogen peroxide (as oxidative stress agent) and glucose starvation (as carbon stress agent)have beenmeasured by quantitative real time polymerase chain reaction. This study offers detailed information on molecular level about glucose sensing, glucose transportation, glucose repression and oxidative stress response pathways and relation between those pathways in S. pombe.