Occurrence of Aerobic and Anaerobic Bacteria in the Consumer End of a Water Distribution System

Hoca S., Ustunturk-Onan M., Ilhan-Sungur E.

WATER ENVIRONMENT RESEARCH, cilt.89, sa.7, ss.598-605, 2017 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 89 Sayı: 7
  • Basım Tarihi: 2017
  • Doi Numarası: 10.2175/106143017x14902968254502
  • Dergi Adı: WATER ENVIRONMENT RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.598-605
  • Anahtar Kelimeler: Biofilm, sulphate reducing bacteria, water distribution systems, viable but not culturable (VBNC), extracellular polysaccharide substances, SULFATE-REDUCING BACTERIA, LEGIONELLA-PNEUMOPHILA, BIOFILM FORMATION, ULCERATIVE-COLITIS, GALVANIZED STEEL, HYDROGEN-SULFIDE, DRINKING-WATER, POTABLE WATER, CORROSION, MICROORGANISMS
  • İstanbul Üniversitesi Adresli: Evet

Özet

In this study, mixed species biofilm formation including sulphate-reducing bacteria (SRB) on polypropylene surface and bacteriology of network water were investigated in a model water distribution system during a nine-month period. Water and biofilm samples were analyzed for the enumeration of aerobic heterotrophic bacteria (AHB), anaerobic heterotrophic bacteria (ANHB) and SRB. The number of live/dead bacteria was also analyzed by epifluorescence microscopy. In addition, extracellular polysaccharide substances (EPS) extraction, carbohydrate analysis and scanning electron microscope observation were performed. A biofilm with heterogeneous structure formed on the polypropylene surface of the model water distribution system. Live/dead staining data indicated that biofilm matured in the first month. It was observed that especially AHB entered into a viable but not culturable state because of the temperature decrease. It was also noted that temperature is an important environmental factor especially for planktonic SRB. The quantity of carbohydrate significantly decreased according to the temperature.