Akademik Veri Yönetim Sistemi
3rd International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2020), Ankara, Türkiye, 19 - 20 Mart 2020, ss.15-22
Fluoride is a natural mineral that found abundantly in the earth's crust. Organic and inorganic fluoride elements are present
in all water and soil sources, and people also take this element by consuming plants and animals as nutrients. Fluorine is in
ionic form in drinking water and thus passes through the intestinal mucosa quickly. Fluoride affects male reproductive
function. Endocrine disrupting effect of fluoride leads to decreased reproductive capacity, testicular spermatozoa
concentration, testicular and auxiliary reproductive organs weights, reduced number of Leydig and Sertoli cells, and
impaired activity of testosterone synthesis and steroidogenic enzymes in Leydig cells. In this study, cytotoxicity, oxidative
damage, and apoptosis were analyzed after Leydig cells were treated with 1.5 ppm sodium fluoride (NaF) for 24 h, in
addition to NaF to ascorbic acid (50 μM) as an antioxidant. The results of cytotoxicity showed that NaF significantly
reduced cell viability and ascorbic acid ameliorate this effect of fluoride. We found that NaF cause oxidative damage via
overproduction of intracellular hydroxyl radical and peroxidation of lipids in cell membrane. Ascorbic acid supplementation
protects NaF-induced oxidative damage on Leydig cells. The findings demonstrated that ascorbic acid has protective effects
of on apoptotic cell death and micronucleus formation caused by NaF in Leydig cells.